gymnonotus uses a network of bat caves geographically segregated hundreds of kilometers apart. Our results indicate the lack of a clear geographic structure with gene flow among all the caves analyzed, suggesting that P. Here, we used ddRADseq data to analyze the genetic structure of Pteronotus gymnonotus across nine bat caves over 700 km. Spatio-temporal reproductive connectivity between distant populations would change our understanding of the use of roosts among bat species in Brazil, and important gaps in knowledge of long-distance bat movements in the country would be filled. However, systematic monitoring of some bat caves in northeastern Brazil shows that they experience strong population fluctuations over short periods of time, suggesting large-scale movements between roosts and a much broader use of the landscape than previously considered. Current knowledge of bat caves in Brazil is still small. These populations play a wider role in ecological interactions, are vulnerable due to their restriction to caves, and have a disproportionate conservation value. 3Bat Lab Finland, Finnish Museum of Natural History, University of Helsinki, Helsinki, Finlandīat caves in the Neotropical region harbor exceptional bat populations (> 100,000 individuals).2Programa de Pós-Graduação em Biologia Animal, Universidade Federal de Pernambuco, Recife, Brazil.1Laboratório de Ciência Aplicada à Conservação da Biodiversidade, Departamento de Zoologia, Universidade Federal de Pernambuco, Recife, Brazil. Fernanda Ito 1,2,3, Thomas Lilley 3, Victoria G.
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